Reference # Publication PDB Chain Mutation(s) Change in Stability Change in Activity Prediction Percentage Change Protherm Status Correct Total Total Enzyme Non Enzyme Total
1 Disulfide bond-deficient azurin mutant 1AZU _ 3ALA/26ALA Decreased Stability NA -4.46 -22.53 None Y 237 Correct Correct
1 Disulfide bond-deficient azurin mutant 1AZU _ 3ALA/26ILE Decreased Stability NA -3.19 -16.11 None Y
17 169
1 Disulfide bond-deficient azurin mutant 1AZU _ 3ALA/25ARG/26ALA/27ARG Decreased Stability NA -5.25 -24.17 None Y Total Correct Incorrect Incorrect
2 In-vitro selection of highly stabilized mutants 1CSQ _ 66LEU/67PRO Increased Stability NA 0.34 4.63 None Y 186 10 41
2 In-vitro selection of highly stabilized mutants 1CSQ _ 2ILE/3SER/46GLN/64LEU/66LEU/67PRO Increased Stability NA 0.6 2.1 None Y
Percentage Percentage
2 In-vitro selection of highly stabilized mutants 1CSQ _ 46LEU/66LEU/67HIS Increased Stability NA 0.58 4.82 None Y Total Incorrect 62.96% 80.48%
2 In-vitro selection of highly stabilized mutants 1CSQ _ 2TYR/3ARG/46LEU Increased Stability NA 0.66 3.7 None Y 51

2 In-vitro selection of highly stabilized mutants 1CSQ _ 2TYR/3ILE/46GLN/64LEU/66LEU/67PRO Increased Stability NA 0.99 4.03 None Y


3 Contribution of tryptophan to the properties of ribotoxin -sarcin 1DE3 A 4PHE Decreased Stability NA 2.89 16.25 None N Total Percentage

3 Contribution of tryptophan to the properties of ribotoxin -sarcin 1DE3 A 51PHE Decreased Stability NA 0.92 7.76 None N 77.97%

3 Contribution of tryptophan to the properties of ribotoxin -sarcin 1DE3 A 4PHE/51PHE Decreased Stability NA 3.21 12.37 None N


4 Reduction of hydrophobicity in bacteriorhodopsin 1DZE A 113GLN Decreased Stability NA -0.26 -2.58 None Y


4 Reduction of hydrophobicity in bacteriorhodopsin 1DZE A 113GLN/116GLN Decreased Stability NA -0.7 -4.64 None Y


4 Reduction of hydrophobicity in bacteriorhodopsin 1DZE A 113GLN/116GLN/120GLN Decreased Stability NA -1.02 -4.61 None Y


4 Reduction of hydrophobicity in bacteriorhodopsin 1DZE A 113GLN/116GLN/120GLN/124GLN Decreased Stability NA -1.71 -5.92 None Y


4 Reduction of hydrophobicity in bacteriorhodopsin 1DZE A 113GLN/116GLN/117ASP/120GLN/124GLN Decreased Stability NA -6.73 -19.42 None Y


5 Mutagenesis of protein phosphatase 1 1FJM A 124ASP Increased Stability Loss of E Act -0.22 -1.47 None N Both N
5 Mutagenesis of protein phosphatase 2 1FJM A 248ASN Increased Stability Loss of E Act -6.4 -28.49 None N Both N
5 Mutagenesis of protein phosphatase 3 1FJM A 96ALA Increased Stability Loss of E Act -0.3 -3.23 None N Both N
5 Mutagenesis of protein phosphatase 4 1FJM A 221SER Increased Stability Loss of E Act 0.78 7.47 None Y Both Y
5 Mutagenesis of protein phosphatase 5 1FJM A 95ALA Increased Stability Loss of E Act 0.17 1.6 None Y Both Y
5 Mutagenesis of protein phosphatase 6 1FJM A 208ALA Increased Stability Loss of E Act -0.23 -2.14 None N Both N
6 Antifungal activity of a rice lipid transfer protein 1FK1 A 45ALA Increased Stability Loss of E Act 3.46 51.78 None Y Both Y
6 Antifungal activity of a rice lipid transfer protein 1FK1 A 46ALA Decreased Stability NA 2.33 18.24 None N


6 Antifungal activity of a rice lipid transfer protein 1FK1 A 72LEU Increased Stability Loss of E Act 5.83 35.16 None Y Both Y
7 C-Reactive Protein Mutants and Pneumococcal Infection of mice 1GNH A 114ALA NA Increased Activity -0.37 28.93 None Y


7 C-Reactive Protein Mutants and Pneumococcal Infection of mice 1GNH A 175ALA NA Loss of E Act 1.42 -16.96 None Y


8 Mutagenesis of hydroxysteroid dehyrogenase 1HXH A 12ALA NA Loss of E Act -0.49 -3.17 None N Enzyme N
8 Mutagenesis of hydroxysteroid dehyrogenase 1HXH A 87ALA Increased Stability Loss of E Act 5.93 40.78 None Y Both Y
8 Mutagenesis of hydroxysteroid dehyrogenase 1HXH A 138ALA NA Loss of E Act 0.03 0.18 None Y Enzyme Y
9 Correlation Between Protein Binding Strength 1L22 _ 83H NA Loss of E Act 2.34 45.16 None Y Enzyme Y
9 Correlation Between Protein Binding Strength 1L22 _ 147H NA Loss of E Act 2.56 48.13 None Y Enzyme Y
9 Correlation Between Protein Binding Strength 1L22 _ 136H Decreased Stability NA -2.08 -22.58 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 138H Decreased Stability NA -7.94 -37.53 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 139H Decreased Stability NA -2.86 -33.92 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 140H Decreased Stability NA -1.41 -20.25 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 141H Decreased Stability NA -1.66 -24.82 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 142H Decreased Stability NA -0.39 -3.24 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 143H Decreased Stability NA -0.63 -12.06 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 144H Decreased Stability NA -1.25 -29.07 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 145H Decreased Stability NA -1.46 -15.34 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 146H Decreased Stability NA -2.14 -19.5 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 148H Decreased Stability NA 0.29 3.25 None N


9 Correlation Between Protein Binding Strength 1L22 _ 149H Decreased Stability NA -3.25 -15.52 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 150H Decreased Stability NA -4.26 -32.48 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 151H Decreased Stability NA -1.34 -14.94 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 152H Decreased Stability NA -0.11 -12.3 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 153H Decreased Stability NA -8.63 -33.18 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 154H Decreased Stability NA -0.11 -1.43 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 155H Decreased Stability NA -0.83 -10.17 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 82H Decreased Stability NA -0.48 -12.79 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 84H Decreased Stability NA -5.35 -28.89 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 85H Decreased Stability NA 1.36 19.29 None N


9 Correlation Between Protein Binding Strength 1L22 _ 86H Decreased Stability NA -2.64 -32.39 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 87H Decreased Stability NA -3.78 -19.86 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 88H Decreased Stability NA -3.92 -23.66 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 89H Decreased Stability NA -1.34 -21.57 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 91H Decreased Stability NA -3.96 -21.88 None Y


9 Correlation Between Protein Binding Strength 1L22 _ 92H Decreased Stability NA 0.52 6.18 None N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2GLY Decreased Stability NA -0.67 -10.28 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2ALA Decreased Stability NA -0.51 -7.75 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2ILE Increased Stability NA 0.58 8.92 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2LEU Decreased Stability NA -0.08 -1.26 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2MET Decreased Stability NA -0.15 -2.24 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2PHE Decreased Stability NA -2.3 -35.09 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2SER Decreased Stability NA -0.65 -9.89 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2TYR Decreased Stability NA -1.04 -15.88 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2ASP Decreased Stability NA -1.26 -19.21 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2ASN Decreased Stability NA -0.82 -12.48 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 2ARG Decreased Stability NA -2.29 -34.96 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74GLY Decreased Stability NA -0.46 -5.62 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74ALA Decreased Stability NA -0.44 -5.39 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74ILE Increased Stability NA -0.78 -9.57 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74LEU Increased Stability NA -0.21 -2.53 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74MET Increased Stability NA -0.42 -5.15 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74PHE Decreased Stability NA 0.43 5.29 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74SER Decreased Stability NA -0.66 -8.19 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74TYR Decreased Stability NA -0.27 -3.31 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74ASP Decreased Stability NA -1.75 -21.58 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74ASN Decreased Stability NA -0.96 -11.86 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 74ARG Decreased Stability NA -1.34 -16.54 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110GLY Increased Stability NA 0.01 0.32 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110ALA Increased Stability NA 0.6 15.73 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110ILE Increased Stability NA 0.04 1.1 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110LEU Increased Stability NA -0.33 -8.52 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110MET Increased Stability NA -0.46 -11.93 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110PHE Decreased Stability NA 0.47 12.32 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110TYR Decreased Stability NA 0.22 5.82 CITED N


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110ASP Increased Stability NA 0.75 19.44 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110ASN Increased Stability NA 0.06 1.59 CITED Y


10 Surface Hydration and stability in lysozyme 1LZ1 _ 110ARG Increased Stability NA 1.79 46.67 CITED Y


11 N-terminal residues and conformational stability of human lysozyme 1LZ1 _ 1MET Decreased Stability NA 0.92 19.46 CITED N


11 N-terminal residues and conformational stability of human lysozyme 1LZ1 _ 1ALA Decreased Stability NA 0.82 17.29 CITED N


12 Mutagensis of Glucanase 1MAC A 101PHE NA Loss of E Act -1.04 -16.03 None N Enzyme N
12 Mutagensis of Glucanase 1MAC A 101TYR NA Loss of E Act 0.26 3.99 None Y Enzyme Y
12 Mutagensis of Glucanase 1MAC A 103ASP NA Loss of E Act -0.12 -1.41 None N Enzyme N
12 Mutagensis of Glucanase 1MAC A 103GLN NA Loss of E Act 1.37 16.81 None Y Enzyme Y
12 Mutagensis of Glucanase 1MAC A 105ASN NA Loss of E Act 1.86 16.07 None Y Enzyme Y
12 Mutagensis of Glucanase 1MAC A 105LYS NA Loss of E Act 1.28 11.04 None Y Enzyme Y
12 Mutagensis of Glucanase 1MAC A 107ASP NA Loss of E Act 0.51 3.41 None Y Enzyme Y
13 Mutations and stability of methylamine dehydrogenase 1MAE L 76ASN Increased Stability NA 0.93 5.88 None Y


13 Mutations and stability of methylamine dehydrogenase 1MAE L 122ALA Increased Stability NA 0.44 1.38 None Y


13 Mutations and stability of methylamine dehydrogenase 1MAE L 122CYS Increased Stability NA 6.6 31.44 None Y


13 Mutations and stability of methylamine dehydrogenase 1MAE L 119PHE Decreased Stability NA -2.62 -9.29 None Y


13 Mutations and stability of methylamine dehydrogenase 1MAE L 119GLU Increased Stability NA -9.85 -60.46 None N


13 Mutations and stability of methylamine dehydrogenase 1MAE L 119LYS Increased Stability NA -5.89 -40.21 None N


14 Human acid-Beta glucosidase and Gaucher disease 1OGS A 370SER Increased Stability Loss of E Act 2.28 15.44 None Y Both Y
14 Human acid-Beta glucosidase and Gaucher disease 1OGS A 394LEU Increased Stability Loss of E Act -1.12 -12.53 None N Both N
14 Human acid-Beta glucosidase and Gaucher disease 1OGS A 463CYS Increased Stability Loss of E Act -1.16 -15.39 None N Both N
14 Human acid-Beta glucosidase and Gaucher disease 1OGS A 469HIS Increased Stability Loss of E Act 0.22 2.15 None Y Both Y
15 Thermostabilization of an Enzyme 1OX7 A 23LEU/140LEU/108ILE Increased Stability NA 1.85 5.98 None Y


15 Thermostabilization of an Enzyme 1OX7 A 23LEU/140LEU Increased Stability NA 1.85 10.44 None Y


15 Thermostabilization of an Enzyme 1OX7 A 23LEU Increased Stability NA -0.65 -5.62 None N


15 Thermostabilization of an Enzyme 1OX7 A 140LEU Increased Stability NA 2.21 13.91 None Y


15 Thermostabilization of an Enzyme 1OX7 A 10THR Increased Stability NA 0.8 15.43 None Y


15 Thermostabilization of an Enzyme 1OX7 A 67GLU Increased Stability NA -4.46 -37.32 None N


15 Thermostabilization of an Enzyme 1OX7 A 69LEU Increased Stability NA 3.34 56.77 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 26HIS NA Loss of E Act 2.38 38.13 None Y Enzyme Y
16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 31THR Decreased Stability NA -0.49 -5.75 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 34LYS Decreased Stability NA -9.48 -48.4 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 93PHE Decreased Stability NA 0.42 4.55 None N


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 116THR Decreased Stability NA -10.14 -32.93 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 116TRP Decreased Stability NA -2.63 -8.53 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 116GLN Decreased Stability NA -13.43 -43.63 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 149LEU Decreased Stability NA 2.48 29.38 None N


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 167TRP NA Loss of E Act -2.49 -13.28 None N Enzyme N
16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 173TRP NA Loss of E Act 2 13.85 None Y Enzyme Y
16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 177ARG Decreased Stability NA -3.8 -34.31 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 201TRP Decreased Stability NA 0.63 3.63 None N


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 223LYS Decreased Stability NA -1.63 -20.13 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 247PHE Decreased Stability NA 2.62 20.81 None N


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 250LYS Decreased Stability NA -3.11 -28.24 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 252THR Decreased Stability NA -1.03 -9.02 None Y


16 Structures of the mutants of prophobilinogen deaminase 1PDA _ 256ASN Decreased Stability NA 0.24 3.31 None N


17 Alanine insertion in lactose permease transmembrane helices 1PV7 A 79ALA Decreased Stability NA 2.61 15.6 None N


17 Alanine insertion in lactose permease transmembrane helices 1PV7 A 83ALA Decreased Stability NA -2.93 -16.58 None Y


17 Alanine insertion in lactose permease transmembrane helices 1PV7 A 87ALA Decreased Stability NA -4.82 -17.13 None Y


17 Alanine insertion in lactose permease transmembrane helices 1PV7 A 96ALA Decreased Stability NA 1.72 14.84 None N


17 Alanine insertion in lactose permease transmembrane helices 1PV7 A 90ALA Decreased Stability NA -2.11 -10.94 None Y


17 Alanine insertion in lactose permease transmembrane helices 1PV7 A 91ALA Decreased Stability NA -3.79 -15.98 None Y


17 Alanine insertion in lactose permease transmembrane helices 1PV7 A 93ALA Decreased Stability NA -0.38 -8.7 None Y


18 Disulfide bonds and stability of acetylcholinesterase 1QO9 A 327CYS/375CYS Increased Stability NA 7.77 34.06 None Y


18 Disulfide bonds and stability of acetylcholinesterase 1QO9 A 354CYS/456CYS Increased Stability NA 1.36 4.74 None Y


18 Disulfide bonds and stability of acetylcholinesterase 1QO9 A 369CYS/476CYS Increased Stability NA 1.82 9.56 None Y


18 Disulfide bonds and stability of acetylcholinesterase 1QO9 A 452CYS/533CYS Decreased Stability NA 1.51 11.14 None N


18 Disulfide bonds and stability of acetylcholinesterase 1QO9 A 464CYS/543CYS Increased Stability NA 2.82 22.11 None Y


19 Stabilizing mutations in subtilisin BPN 1SBT _ 41ALA/50PHE/73LEU/206TRP/217LYS/218SER/221CYS/271GLU Increased Stability NA 2.39 3.11 None Y


19 Stabilizing mutations in subtilisin BPN 1SBT _ 2LYS/3CYS/5SER/43ASN/50PHE/73LEU/206CYS/217LYS/218SER/271GLU Increased Stability NA 2.57 3.13 None Y


20 Salt Bridges and stability of subtilisin BPN 1SBT _ 271GLU Decreased Stability NA -0.1 -1.42 None Y


20 Salt Bridges and stability of subtilisin BPN 1SBT _ 51LYS Decreased Stability NA -3.37 -35.21 None Y


20 Salt Bridges and stability of subtilisin BPN 1SBT _ 164ARG Decreased Stability NA -2.44 -21.27 None Y


21 Mutagenesis of the unfolding region of ribonuclease A 2AAS _ 35SER Decreased Stability NA -1.04 -15.73 None Y


21 Mutagenesis of the unfolding region of ribonuclease A 2AAS _ 35ALA Decreased Stability NA -1.53 -8.02 None Y


21 Mutagenesis of the unfolding region of ribonuclease A 2AAS _ 46TYR Decreased Stability NA -0.85 -4.11 None Y


21 Mutagenesis of the unfolding region of ribonuclease A 2AAS _ 31ALA/33SER Decreased Stability NA 8.74 69.54 None N


21 Mutagenesis of the unfolding region of ribonuclease A 2AAS _ 35SER/46TYR Decreased Stability NA -1.08 -4.38 None Y


21 Mutagenesis of the unfolding region of ribonuclease A 2AAS _ 35ALA/46TYR Decreased Stability NA -1.21 -4.93 None Y


21 Mutagenesis of the unfolding region of ribonuclease A 2AAS _ 31ALA/33SER/46TYR Decreased Stability NA 7.64 25.91 None N


22 Radical stability in ribonucleotide reductase from E-coli 2ALX A 212TRP Decreased Stability NA -1.62 -7.52 None Y


22 Radical stability in ribonucleotide reductase from E-coli 2ALX A 234ASN Decreased Stability NA -5.41 -34.1 None Y


23 Evolutionally conserved aspartic acid residues in human glutathione 6GSS A 57ALA Decreased Stability NA 2.54 27.26 None N


23 Evolutionally conserved aspartic acid residues in human glutathione 6GSS A 98ALA Decreased Stability NA 1.07 16.61 None N


23 Evolutionally conserved aspartic acid residues in human glutathione 6GSS A 152ALA Decreased Stability NA 5.17 43.54 None N


24 Hydrophobic core mutations and stability 1B2X A 14ALA Decreased Stability NA -3.07 -12.62 None Y


24 Hydrophobic core mutations and stability 1B2X A 51VAL Decreased Stability NA -0.28 -2.46 None Y


24 Hydrophobic core mutations and stability 1B2X A 76ALA Decreased Stability NA -2.22 -12.78 None Y


24 Hydrophobic core mutations and stability 1B2X A 76VAL Decreased Stability NA 0.46 2.67 None N


24 Hydrophobic core mutations and stability 1B2X A 88ALA Decreased Stability NA -4.83 -18.47 None Y


24 Hydrophobic core mutations and stability 1B2X A 88VAL Decreased Stability NA -1.29 -4.93 None Y


24 Hydrophobic core mutations and stability 1B2X A 89VAL Decreased Stability NA -0.79 -3.08 None Y


24 Hydrophobic core mutations and stability 1B2X A 96ALA Decreased Stability NA -2.55 -13.76 None Y


24 Hydrophobic core mutations and stability 1B2X A 96VAL Decreased Stability NA -1.05 -5.65 None Y


24 Hydrophobic core mutations and stability 2CI2 I 39VAL Decreased Stability NA -0.46 -2.3 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 48ALA Decreased Stability NA -1.99 -16.42 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 48VAL Decreased Stability NA -0.44 -3.62 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 48ALA/76VAL Decreased Stability NA -2.18 -7.57 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 66ALA Decreased Stability NA -2.44 -13.86 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 68ALA Decreased Stability NA -2.66 -13.34 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 70ALA Decreased Stability NA -1.87 -11.79 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 76VAL Decreased Stability NA -0.19 -1.15 CITED Y


24 Hydrophobic core mutations and stability 2CI2 I 76ALA Decreased Stability NA -2.14 -12.81 CITED Y


24 Hydrophobic core mutations and stability 1EY0 A 141ALA Decreased Stability NA 0.25 5.8 None N


24 Hydrophobic core mutations and stability 1EY0 A 99ALA Decreased Stability NA -4.18 -18.84 None Y


24 Hydrophobic core mutations and stability 1EY0 A 74ALA Decreased Stability NA -3.33 -14.52 None Y


24 Hydrophobic core mutations and stability 1EY0 A 72VAL Decreased Stability NA -0.19 -0.81 None Y


24 Hydrophobic core mutations and stability 1EY0 A 34ALA Decreased Stability NA -4.19 -17.33 None Y


24 Hydrophobic core mutations and stability 1EY0 A 66ALA Decreased Stability NA -3.76 -13.94 None Y


24 Hydrophobic core mutations and stability 1EY0 A 23ALA Decreased Stability NA -4.55 -15.23 None Y


24 Hydrophobic core mutations and stability 1EY0 A 25ALA Decreased Stability NA -4.35 -14.28 None Y


24 Hydrophobic core mutations and stability 1EY0 A 92VAL Decreased Stability NA -1.32 -4.29 None Y


24 Hydrophobic core mutations and stability 1EY0 A 92ALA Decreased Stability NA -6.1 -19.78 None Y


24 Hydrophobic core mutations and stability 1EY0 A 36ALA Decreased Stability NA -1.97 -10.39 None Y


24 Hydrophobic core mutations and stability 1EY0 A 72VAL/113ALA Decreased Stability NA 0 0.01 None N


24 Hydrophobic core mutations and stability 1EY0 A 72VAL/85ALA Decreased Stability NA -0.64 -2.37 None Y


24 Hydrophobic core mutations and stability 1EY0 A 66LEU Decreased Stability NA 0.33 1.22 None N


24 Hydrophobic core mutations and stability 1EY0 A 23PHE Decreased Stability NA -0.15 -0.49 None Y


24 Hydrophobic core mutations and stability 1EY0 A 117GLY/124LEU/128ALA Increased Stability NA 5.04 27.08 None Y


24 Hydrophobic core mutations and stability 1EY0 A 41ILE/117GLY/124LEU/128ALA Increased Stability NA 6.7 23.12 None Y


24 Hydrophobic core mutations and stability 1EY0 A 33VAL/41ILE/117GLY/124LEU/128 ALA Increased Stability NA 7.71 19.81 None Y


24 Hydrophobic core mutations and stability 1EY0 A 41ILE/59ALA/117GLY/124LEU/128ALA Increased Stability NA 7.68 20.54 None Y


24 Hydrophobic core mutations and stability 1IGV A 6VAL Decreased Stability NA 0.01 0.08 CITED N


24 Hydrophobic core mutations and stability 1IGV A 23ALA Decreased Stability NA -3.45 -18.86 CITED Y


24 Hydrophobic core mutations and stability 1IGV A 28ALA Decreased Stability NA -5.02 -19.84 CITED Y


24 Hydrophobic core mutations and stability 1IGV A 61ALA Decreased Stability NA -1.57 -9.12 CITED Y


24 Hydrophobic core mutations and stability 1IGV A 66TRP Decreased Stability NA -2.94 -9.53 CITED Y


24 Hydrophobic core mutations and stability 1IGV A 66ALA Decreased Stability NA -4.48 -14.51 CITED Y


24 Hydrophobic core mutations and stability 1IGV A 70LEU Decreased Stability NA 1.69 8.08 CITED N


24 Hydrophobic core mutations and stability 1IGV A 73VAL Decreased Stability NA -0.29 -1.8 CITED Y


24 Hydrophobic core mutations and stability 1IGV A 10ALA Decreased Stability NA -1.9 -10.07 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 46 ALA Decreased Stability NA -4.05 -16.76 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 71 ILE Decreased Stability NA 0.78 4.33 CITED N


24 Hydrophobic core mutations and stability 1L63 _ 1 71 MET Decreased Stability NA -2.23 -9.5 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 71 PHE Decreased Stability NA -0.95 -3.81 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 99 ALA Decreased Stability NA -5.17 -15.6 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 100 ALA Decreased Stability NA -3.13 -13.99 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 118 ALA Decreased Stability NA -3.7 -13.68 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 121 ALA Decreased Stability NA -6.6 -22.66 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 133 ALA Decreased Stability NA -0.48 -8.43 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 153 VAL Decreased Stability NA -0.87 -3.47 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 153 MET Decreased Stability NA -3.03 -11.5 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 153 LEU Increased Stability NA 0.31 1.15 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 153 ILE Decreased Stability NA -0.53 -2.1 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 153 ALA Decreased Stability NA -5.04 -19.4 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 17 ALA Decreased Stability NA -3.61 -25.01 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 27 ALA Decreased Stability NA -4.05 -19.97 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 50 ALA Decreased Stability NA -1.71 -15.41 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 58 ALA Decreased Stability NA -4.13 -22.66 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 78 ALA Decreased Stability NA -2.28 -14.34 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 87 ALA Decreased Stability NA -1.81 -9.31 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 103 ALA Decreased Stability NA -2.15 -11.79 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 111 ALA Decreased Stability NA -2.32 -12.8 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 67 ALA Decreased Stability NA -2.48 -17.62 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 104 ALA Decreased Stability NA -2.68 -17.46 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 66 ALA Decreased Stability NA -2.75 -14.38 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 84 ALA Decreased Stability NA -3.23 -17.26 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 91 ALA Decreased Stability NA -2.74 -15.61 CITED Y


24 Hydrophobic core mutations and stability 1L63 _ 1 71 ALA Decreased Stability NA -3.27 -14.62 CITED Y

























80 CITED